The somatic proteins of Toxocara canis larvae and excretory-secretory products revealed by proteomics
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چکیده
منابع مشابه
Proteinases in Excretory-Secretory Products of Toxocara canis Second-Stage Larvae: Zymography and Modeling Insights
Components released in excretory-secretory products of Toxocara canis larvae (TES) include phosphatidylethanolamine-binding proteins (TES26), mucins (TES120, MUC2-5), and C-type lectins (TES32, TES70) and their biochemical, immunological, and diagnostic properties have been extensively studied albeit proteinase activities towards physiological substrates are almost unknown. Proteolytic activiti...
متن کاملSandwich ELISA detection of excretory-secretory antigens of Toxocara canis larvae using a specific monoclonal antibody.
We produced a new monoclonal antibody (mAb) to the excretory-secretory (ES) antigens of Toxocara canis larvae. The mAb (IgG1) reacts specifically with the 120 kDa protein of many ES molecules and does not have any cross-reactivity with adult T. canis antigens. Sandwich ELISA to detect the ES antigens was performed using the mAb and rabbit polyclonal antiserum. The lower limit for the detection ...
متن کاملIsolation and identification of excretory-secretory and somatic antigens from the Oestrus ovis larvae by SDS-PAGE and immunoblotting
Oestrus ovis is an economically important parasite of small ruminants and a zoonotic parasite with many reports of ophthalmomyiasis in human from Iran and other countries. The aim of the peresent studywas the isolation and identification of excretory-secretory (ES) and somatic (S) antigens of O. ovis second and third stage larvae (L2, L3) collected from Arabi sheep breeds loca...
متن کاملToxocara enzyme linked immunosorbent assay titres using T canis excretory-secretory products as antigen
reports have emphasised the severity of neurological and systemic disorder in patients with toxocaral meningoencephalitis.1 2 " Apart from neck stiffness our patient was surprisingly well with no evidence of encephalitis. Her diagnosis might have been that of an atypical viral meningitis had Romanovsky staining not been performed. We think that this investigation may be useful in culture negati...
متن کاملEvaluate Toxocara Canis Excretory-Secretory Antigens in Experimental Allergic Encephalomyelitis (EAE)
BACKGROUND: Toxocara canis is the most prevalent intestinal roundworm of canid species. OBJECTIVES: This study aims to evaluate the effects of Toxocara canis excretory-secretory antigens (TcES Ag) on modulating the immune system in Experimental Allergic Encephalomyelitis (EAE) model. METHODS: Adult worms of T.canis were collected from dogs to obtain excretory-secretory antigens. Female C57BL/6m...
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ژورنال
عنوان ژورنال: Veterinary Parasitology
سال: 2018
ISSN: 0304-4017
DOI: 10.1016/j.vetpar.2018.06.015